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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
18/10/2018 |
Data da última atualização: |
18/10/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F.; RAGHOTHAMA, K. G. |
Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; Purdue University. |
Título: |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 17, n. 4, p. 1-9, 2018. |
DOI: |
10.4238/gmr18036 |
Idioma: |
Inglês |
Notas: |
Article: gmr18036. |
Conteúdo: |
Phosphate (Pi) unavailability is a growth-limiting factor for plants. Under Pi-limited conditions, plants activate molecular mechanisms for better acquisition and utilization of this nutrient. In maize, changes in the expression pattern of several Pi starvation-induced genes, including the A1 coding for dihydroflavonol 4-reductase (DFR) involved in anthocyanin biosynthesis, were identified through microarray analysis. In order to elucidate the molecular determinants with a potential role in P use efficiency, we carried out a study on gene expression analysis of the A1 phosphate responsive gene by northern blot analysis of total RNA from maize genotypes contrasting for Pi efficiency. Two Pi-efficient (L-03 and L-161-1) and five inefficient (L-11, L-16, L-22, L-53, and L-5046) genotypes of maize were grown for 15 days in hydroponic culture in the presence (250 ,uM Pi) or absence (0 ,uM Pi) of phosphate. All genotypes showed an increase in anthocyanin accumulation in roots in the absence of Pi (0 ,uM Pi). The Pi-efficient genotype L-36 and the Pi-inefficient genotypes L-16, L22, and L-5046 showed the highest levels of anthocyanin accumulation. The A1 gene exhibits temporal and spatial expression patterns associated with Pi deficiency. Although there were differences in the expression profile of Pi starvation induced genes, no consistent expression patterns could be associated with either Pi-efficient or Pi-inefficient genotypes. It appears that Pi efficiency in tropical maize is a complex trait mediated by a coordinated action of genes that are either induced or suppressed in response to Pi-deficiency. MenosPhosphate (Pi) unavailability is a growth-limiting factor for plants. Under Pi-limited conditions, plants activate molecular mechanisms for better acquisition and utilization of this nutrient. In maize, changes in the expression pattern of several Pi starvation-induced genes, including the A1 coding for dihydroflavonol 4-reductase (DFR) involved in anthocyanin biosynthesis, were identified through microarray analysis. In order to elucidate the molecular determinants with a potential role in P use efficiency, we carried out a study on gene expression analysis of the A1 phosphate responsive gene by northern blot analysis of total RNA from maize genotypes contrasting for Pi efficiency. Two Pi-efficient (L-03 and L-161-1) and five inefficient (L-11, L-16, L-22, L-53, and L-5046) genotypes of maize were grown for 15 days in hydroponic culture in the presence (250 ,uM Pi) or absence (0 ,uM Pi) of phosphate. All genotypes showed an increase in anthocyanin accumulation in roots in the absence of Pi (0 ,uM Pi). The Pi-efficient genotype L-36 and the Pi-inefficient genotypes L-16, L22, and L-5046 showed the highest levels of anthocyanin accumulation. The A1 gene exhibits temporal and spatial expression patterns associated with Pi deficiency. Although there were differences in the expression profile of Pi starvation induced genes, no consistent expression patterns could be associated with either Pi-efficient or Pi-inefficient genotypes. It appears that Pi efficiency in tropical maize i... Mostrar Tudo |
Palavras-Chave: |
Estresse abióco; Expressão genética. |
Thesagro: |
Antocianina; Solo Ácido. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184692/1/Expression-analysis.pdf
|
Marc: |
LEADER 02368naa a2200217 a 4500 001 2097743 005 2018-10-18 008 2018 bl uuuu u00u1 u #d 024 7 $a10.4238/gmr18036$2DOI 100 1 $aVASCONCELOS, M. J. V. de 245 $aExpression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency.$h[electronic resource] 260 $c2018 500 $aArticle: gmr18036. 520 $aPhosphate (Pi) unavailability is a growth-limiting factor for plants. Under Pi-limited conditions, plants activate molecular mechanisms for better acquisition and utilization of this nutrient. In maize, changes in the expression pattern of several Pi starvation-induced genes, including the A1 coding for dihydroflavonol 4-reductase (DFR) involved in anthocyanin biosynthesis, were identified through microarray analysis. In order to elucidate the molecular determinants with a potential role in P use efficiency, we carried out a study on gene expression analysis of the A1 phosphate responsive gene by northern blot analysis of total RNA from maize genotypes contrasting for Pi efficiency. Two Pi-efficient (L-03 and L-161-1) and five inefficient (L-11, L-16, L-22, L-53, and L-5046) genotypes of maize were grown for 15 days in hydroponic culture in the presence (250 ,uM Pi) or absence (0 ,uM Pi) of phosphate. All genotypes showed an increase in anthocyanin accumulation in roots in the absence of Pi (0 ,uM Pi). The Pi-efficient genotype L-36 and the Pi-inefficient genotypes L-16, L22, and L-5046 showed the highest levels of anthocyanin accumulation. The A1 gene exhibits temporal and spatial expression patterns associated with Pi deficiency. Although there were differences in the expression profile of Pi starvation induced genes, no consistent expression patterns could be associated with either Pi-efficient or Pi-inefficient genotypes. It appears that Pi efficiency in tropical maize is a complex trait mediated by a coordinated action of genes that are either induced or suppressed in response to Pi-deficiency. 650 $aAntocianina 650 $aSolo Ácido 653 $aEstresse abióco 653 $aExpressão genética 700 1 $aFIGUEIREDO, J. E. F. 700 1 $aRAGHOTHAMA, K. G. 773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 17, n. 4, p. 1-9, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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Registros recuperados : 19 | |
1. | | VASCONCELOS, M. J. V.; RAGHOTHAMA, K. G. Análise da expressão de genes induzidos por fósforo em genótipos contrastantes de milho, selecionados para eficiência do uso de fósforo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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12. | | NAGY, R.; VASCONCELOS, M. J. V.; ZHAO, S.; McELVER, J.; BRUCE, W.; AMRHEIN, N.; RAGHOTHAMA, K. G.; BUCHER, M. Differential regulation of five Pht1 phosphate transporters from maize (Zea mays L.). Plant Biology, New York, v. 8, n. 2, p. 186-197, 2006.Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Milho e Sorgo. |
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14. | | RAGHOTHAMA, K. G.; KARTHIKEYAN, A. S.; JAIN, A.; RAMAIAH, M.; VASCONCELOS, M. J. V.; WAND, J.; CAO, A.; BALLACHANDA, D. Molecular regulation of phosphate starvation responses in plants. In: INTERNATIONAL SYMPOSIUM ON PHOSPHORUS DYNAMICS IN THE SOIL-PLANT CONTINUUM, 3., 2006, Uberlândia. Proceedings... Sete Lagoas: Embrapa Milho e Sorgo, 2006. p. 86.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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16. | | VASCONCELOS, M. J. V. de; JAIN, A.; FIGUEIREDO, J. E. F.; OLIVEIRA, M. F. de; TRINDADE, R. dos S.; YUGANDHAR, P.; RAGHOTHAMA, K. G. Gaspé Flint corn as a seed-to-seed model to study the effect of phosphorus on maize growth and development. Genetics and Molecular Research, v. 23, n. 1, gmr19213, 2024.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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17. | | VASCONCELOS, M. J. V. de; SCHAFFERT, R. E.; OLIVEIRA, M. F. de; JAIN, A.; FIGUEIREDO, J. E. F.; RAGHOTHAMA, K. G. Isolation of high-affinity phosphate transporters SbPT1 AND SbPT2 in Sorghum bicolor and their characterization in contrasting genotypes. Genetics and Molecular Research, v. 20, n. 2, gmr18717, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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19. | | COELHO, G. T. C. P.; CARNEIRO, N. P.; KARTHIKEYAN, A. S.; RAGHOTHAMA, K. G.; SCHAFFERT, R. E.; BRANDAO, R. L.; PAIVA, L. V.; SOUZA, I. R. P. de; ALVES, V. M.; IMOLESI, A.; CARVALHO, C. H. S.; CARNEIRO, A. A. A phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation. Plant Molecular Biology Reporter, Athens, v. 28, p. 717-723, 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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Registros recuperados : 19 | |
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